PCR Technology: Current Innovations - No Cost Library

PCR Technology: Current Innovations - 3rd Edition

   Author(s): Tania Nolan, Stephen A. Bustin  

Publisher: CRC Press, Year: 2013

                                           

 Description: 

In some ways, the simplicity of PCR as a molecular technique is responsible for the enormous amount of innovation that surrounds it, as researchers continually think of new ways of tweaking, adapting, and re-formulating concepts and applications. PCR Technology: Current Innovations, Third Edition is a set of innovative approaches, observations, and viewpoints that provide a critical and timely point of reference for anyone wishing to use this technology.

In this forward-thinking volume the topics include:

Cleansing and handling of PCR templates

Impact of oligonucleotide manufacture and purification on PCR behaviour

Optimum composition with buffer

Sample Options

qPCR assays design and optimisation

Issues relating to the development and refinement of the instruments

Efficient reaction variability controls to defend against uncertainties

The book covers all aspects of PCR and PCR in real time, and contains detailed protocols that make it suitable as both a reference and instruction manual. Each chapter provides detailed guidance as well as helpful hints and tips provided by authors who are recognized experts in their fields. The book also provides information on new developments in the PCR arena, in addition to descriptions of current technology and best practices.

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Book Reviews:

"This ... edition contains up-to - date details on PCR technologies and implementations and includes a range of commonly used techniques and solutions for both qualitative and quantitative questions. It definitely presents easy-to-follow, step-by - step procedures and discusses the conditions controlling PCR reaction outcomes ... ... [A] fundamental reference for scientists new to PCR technology and a source of up-to - date applications for those familiar with the method .... I strongly recommend this book as a useful tool for hospital scientists who may use PCR technology in their work; or for scientists.